Professor, Department of Horticulture,
4017 ALS, Oregon  State University, Corvallis, OR 97331-7304., (541) 757-7456, (541)-737-5480, FAX (541) 737-3479

POSITION DESCRIPTION My job description is to: guide and carry out, on the Main Campus, a laboratory oriented physiological, biochemical, biophysical, biochemical-plant pathological, gene expression research to identify and characterize plant germplasm1 (Corylus, Fragaria, Mentha, Prunus, Pyrus, Rubus, Ribes, Vaccinium, Vitis, and others). The objective is to provide basic information to support Oregon's exceptionally diverse agriculture (1995 CSREES report). My FTE distribution is 0.9 Research, 0.1 Instruction. Research (0.9).

My Experiment Station Project (ORE 00322, renewed and approved 1995) has four specific objectives

1) Continue basic biochemical and biophysical characterization of plant germplasm

2) Identification investigations will emphasize localization of desirable germplasm traits through automated biophysical/biochemical instrumentation.

3) Develop instrumentation based technologies

4) Crop genera for which appropriate traits will be sought: will include: Corylus, Fragaria, Mentha, Populus, Prunus, Pyrus, Rubus, Ribes, Vaccinium, Vitis, related genera and other crop genera of Oregon and the Northwest region.

Project ORE00322 is also described verbatim ac litteratim2 in items 24 (Objectives) and 25 (Approach) of US Department of Agriculture Research unit/project description-Research Resume of April 1, 1995): 2 Item 24. "Essential objectives are to: A. Characterize germplasm (Horticultural Emphasis) sources with traits useful to crops and industries of Oregon and Northwest region, and B. identify (fingerprint) these traits by various biochemical and biophysical means." Item 25. "Continue basic biochemical and biophysical characterization of plant germplasm. Special emphasis will be place on characterizing the resistance of hazel (filbert trees) to diverse pathogens and other challenges, photosynthetic and other metabolic adaptations to production environments, and characterizing optical traits of crops that have potential use in remote and robotic sensing for germplasm and other agricultural objectives. Identification investigations will concentrate on development of computer enhanced equipment that will yield rapid through put high resolution screens for valuable germplasm traits including those related to crop bioengineering. Aid grower associations with the development of instrumentation technology to promote grower productivity." Instruction (0.1) For the period September 1983 to August 1988 my FTE was 100% research, 0% instruction. However this was changed in 1988 to 90% research and 10% instruction

DESCRIPTION OF AREA OF SPECIALIZATION My specialization investigates plant germplasm-physiology, -biochemistry and -biophysics. This specialization is important to agriculture. Basic principles of germplasm investigations include: (I) practical, but long term, goals. (II) These goals are often, only reachable, with advanced instrumentation. (III) germplasm research is not a reductionist approach in which one phenomena, in one group of plants, is researched to the maximum extent possible. Germplasm research concerns comparisons of specific abilities between different plants. (IV) Variable search processes: germplasm research uses many scientific specializations to seek useful abilities of plants. Thus germplasm investigations do not always have the ordered aspect of more reductionist sciences, or the intense goal oriented aspects of field research. Germplasm research seems to violate concepts of reductionist discipline by `hopping around cherry picking' from species to species using methods chosen or developed as necessity dictates. Yet, this is what we must do to seek, culled from the fertile plains, swamps, woods, barrens and jungles of the plant world, germplasm activities of interest and utility.

Objectives of specialization We need to find the most efficient and best suited plant activities that can be used, to breed or bioengineer better crop plants. US agriculture is now the most advanced and competitive in the world, this could change. Challenges of growing populations, ever-advancing technology, more open world markets, new competing sources of products in and for the Pacific Rim countries mean US agriculture must be even more competitive and technologically advanced to survive. This is especially true in northwestern states, such as Oregon, which need to maintain their traditionally extensive trade relations with Pacific Rim countries. To survive we need a continuous supply of better producing, more competitive, crop plants. To better crop plants we need better germplasm.


(a) A patent for an absorbance spectrophotometer/fluorescent imaging device to determine physiology, plant pathology, and water content and water stress was filed, as number 60/010,025 with the US Patent and Trademark Office on January 12, 1996. This device has attracted considerable attention in the press from Scientific American (July 1996, p.37) to the Times of London (August 4, 1996) to Analytical Chemistry (News Section Jan 1, 1996, p15A) and including phone interviews with The Independent (London), Editorial Abril (Brazilian magazine group), and Biophotonics. A number of contacts have been. From this work applied concepts useful for industrial lumber screening and NASA remote sensing of vegetation were developed.

(b) The in vivo thermodynamics of CO2 fixation in Crassulacean acid metabolism (CAM) plants were explored. Some CAM plants have carbon fixing systems more chill tolerant than those of corn (Zea mays). This provides germplasm to bioengineer more chill resistant sweet corn for Oregon (publication numbers). This is an important finding because cold temperatures limit the planting dates of sweet corn in Oregon, and the bioengineering substitution of such chill resistant CAM enzymes for their corresponding Zea analogues, would allow earlier planting and more assured production. This gave a novel theoretical rationale to consider the Crassulacean Acid Metabolism photosynthetic path as ancestral to both C-3 and C-4 paths (Ning et al., 1994b).

(c) Other germplasm technologies developed and used include: a spectroscopic technology was developed to identify various plant pigments in vivo . The basic science of this was accepted and with this technology we explored June yellows a non-infectious seed carried condition of strawberries (Fragaria) responsible for the loss of many promising cultivars. The National Clonal Germplasm Repository at Corvallis keeps a world collection of Fragaria. June yellows involves changes in expression of chlorophyll protein complexes inherited from F. virginiana and F. chiloensis. Of the (~400) strawberry cultivars kept at this Repository, 12 showed June yellows. However, these affected cultivars were found to have been used in the breeding of most, if not all, commercial cultivars. A known source of this condition is the cultivar Howard 17. Spectrophotometric examination of the leaves of these plants followed by statistical analysis provided a way to distinguish June yellows inherited from Howard 17 from that inherited from cultivar Aberdeen a distinct, new, and unsuspected source of this condition (see publications in vitae). The field lethal aurea trait of hazel (Corylus) cultivar `Barcelona' progeny was found to be light harvesting chlorophyll protein complex lesions (see publications in vitae). Since the genus Corylus is so rich in germplasm diversity (see publications in vitae) indicating relatively only recent selective for cultivation, since the aurea mutation a common recessive gene which when expressed phenotypically grows well under canopy this is probably a gene related to survival of the wild accessions in understorey forest conditions. An in vivo method was developed to characterize the genetics of intensity of anthocyanin color of hazel leaves. Results suggest a second gene for red leaf color (refereed publications in vitae). This use is distinct and from what is usually done in similar genetic studies in that the evaluation of the in vivo intensity of color and the nature of the chromophore were measured, and also it seems to be a new and novel approach.

A novel quantification of species diversity was developed. Germplasm accessions were electrophoretically fingerprinted by peroxidase, esterase and acid phosphatase isozymic patterns (see publications and report on Cooperative Agreement to USDA for verification). More than 900 pear (Pyrus), 80 hazel (Corylus), and all available quince (Cydonia) accessions were electrophoretically fingerprinted by peroxidase, esterase and acid phosphatase isozymic patterns.


(e) First demonstration of light activation of RuBPCase and the demonstration of the phosphohydroxypyruvate and phosphoserine pathway in photosynthesis (1976) (f) Other investigation included: Determination of the kinetic equilibria and thus the physiological function of phosphoenolpyruvate carboxykinase in CAM plant photosynthesis (4), working out the optimal greenhouse conditions for Jade production (6), enzyme development in germinating peach buds (10,11), demonstration of site of Kethane sensitization of cotton to Verticillium wilt (9). Ultra-microassay for galactose in Helminthosporoside, pathogen generated toxin of sugarcane (16).

Name:  Laurence (Larry) S. Daley               Rank:  Associate Professor
Department:  Horticulture                Speciality: Biochemistry & Biophysics of Germplasm
Date Hired at OSU:  September 1983             Date of Last: Promotion June 1997
FTE: Instruction 0.10                          Research 0.90

Degree   Major                     Institution                Department
B.S.A.   Fruit Crops            Univ. of Florida,      Fruit Crops Dept.
M.S.A.   Fruit Crops            Univ. of Florida       Fruit Crops Dept.
Ph.D.    Plant Physiology       U.C. Davis             Biochemistry and Biophysics

Position      Specialization                            Department and Institution                 Years
Postdoct.    Photosynthesis                             Biology, Queen's Univ. Ontario           1974-75
Postdoct.    Physiological Plant Pathology              Boyce Thompson Research Institute,
								        Yonkers, NY      				 1975-77
Postdoct.    Plant Physiology/Biochemistry Horticulture Univ. of Georgia                         1977-78
NSF Visiting Associate Professor * Plant Pathology 	  Montana State Univ.                (summer) 1981
Assistant Research Professor Ad interim Biological Sciences, North Texas Univ.                   1979-80
Associate Professor Ad interim                          Biology, East Texas State Univ.          1980-82
NSF Visiting Associate  Professor, Molec * Plant Biology, U.C. Berkeley
(summer) 1982 Research Associate Professor Chemistry,   North Texas Univ.                        1982-83
Visiting Associate Professor*                           DOD Aberdeen Proving
Grounds MA-              											 (summer) 1983

Assistant Professor     Germplasm      Horticulture, Oregon State Univ.                  		 1983-87
Associate Professor     Germplasm      Horticulture, Oregon State Univ.					 1987-97
Professor               Germplasm      Horticulture, Oregon State Univ.                          1997---

REFEREED JOURNAL ARTICLES #Indicates Larry Daley as corresponding author.
53. Bowyer, W.J. , Ning, Li, L.S. Daley#, G.A. Strobel, G.E. Edwards and
J.B. Callis. In vivo fluorescent imaging for detection of damage to leaves
by fungal phytotoxins.(accepted).
52. Ning, Li, B.E. Petersen, G. E. Edwards, L.S. Daley#, and G.A. Strobel,
and J.B. Callis. 1997.  Recovery of digital information stored in living
plant leaf photosynthetic apparatus as fluorescence signals. Applied
Spectroscopy  51(1) 1- 9.
51. Ning, Li, W.J. Bowyer, A.M. Chozinski, T. Buban, A. Azarenko, G.E.
Edwards, L.S. Daley#, and G.A. Strobel, and J.B. Callis. 1997. Five novel
applications of imaging visible and short near infrared spectrophotometry
and fluorometry in the plant sciences. Part II. Non-invasive in vivo
applications. Spectroscopy  12(1 )  37-46.
50. Ning, Li, W.J. Bowyer, A. M. Chozinski, T. Buban, A. Azarenko, G. E.
Edwards, L.S. Daley#, and G.A. Strobel, and J.B. Callis. 1996. Five novel
applications of imaging visible and short near infrared spectrophotometry
and fluorometry in the plant sciences. Part I. Photographic and
histological applications. Spectroscopy 11 (9) 44-49.
49. Ning, Li, W.J. Bowyer, L.S. Daley#, E.H. Piepmeier, G.A. Strobel, and
J.B. Callis, 1996. Spectroscopic imaging of water in living plant leaves.
Part II: Challenges, uses and advantages of in vivo absorbance methods for
the analysis of biological materials. Spectroscopy 11(4) 68-74.
48. Ning, Li, W.J. Bowyer, L.S. Daley#, E.H. Piepmeier, G.A. Strobel, and
J.B. Callis, 1996.  Spectro   scopic imaging of water in living plant
leaves. Part I: "Rayleigh" corrections and significance for Beer's law
based in situ quantification part II,  Spectroscopy11 (3), 34-44 and
47. Ning, L., G.E. Edwards, G.A. Strobel, L.S. Daley# and J.B. Callis,
1995 Imaging fluorometer to detect pathological and physiological change
in plants. Applied Spectroscopy 49(10), 1381-1389 and cover (1995).
46. Gardea, A.A., Y.M. Moreno, A.N. Azarenko, P.B. Lombard, L.S. Daley and
R.S. Criddle. 1994. Changes in metabolic properties of grape buds during
development. J. Amer. Soc. Hort. Sci. 119: 756-760.
45. Ning, L., L.S. Daley#, Zhao Lu and J. B. Callis. 1994a. Imaging of the
interior of Tradescantia zebrina Bosse leaves by optical cross correlation
interferometry. Biochem. Biophys. Res. Communic. 205(1): 638-644.
44. Ning, L., R. Ozanich, L.S. Daley# and J.B. Callis, 1994b. Construction
of an imaging spectrophotome   ter and its application to plant sciences.
Spectroscopy 9(7): 41-48.
43. Eskelsen, S.R., G.D. Crabtree, R.B. Boone, G.M. Volk, L. Ning and L.S.
Daley#. 1994. Visible spec   troscopy of herbicides: monitoring in vivo
effects and modeling off-target movement. Spectroscopy 7(2): 34-40.
42. Feng, W., L. Ning, L.S. Daley#, Y. Moreno, A. Azarenko and R.S.
Criddle. 1994a. Determination of effective temperature minima for CAM
carboxylation in diverse plants by scanning microcalorime   try. Plant
Physiol. Biochem 32: 319-330.
41. Feng, W., L. Ning, L.S. Daley#, Y. Moreno, A. Azarenko and R.S.
Criddle. 1994b. Theoretical fitting of energetics of CAM path to
calorimetric data. Plant Physiol. Biochem 32:591-598.
40. Gardea, A.A., L.S. Daley#, R.L. Kohnert, A.H. Soeldner, L. Ning, P. B.
Lombard and A.N. Azarenko. 1994. Proton NMR signals associated with eco-
and endodormancy in winegrape buds. Scientia Hort. 56: 339-358.
39. Gardea, A.A., L.S. Daley#, R.L. Kohnert, A.H. Soeldner, L. Ning, P. B.
Lombard and A.N. Azarenko. 1993. In situ proton NMR Methods in plant
science following the development of winegrape buds. Spectroscopy 8(1):
38. Dixon, A.R., R.B. Boone, A. Gardea, L. S. Daley and T.L. Righetti.
1990.  Analysis of electrophoresis isozyme patterns using
microcomputer-based imaging system. HortScience 25(8): 961-964.
37. Yoder B.J. and L.S. Daley#. 1990. Development of in vivo chlorophyll a
and -b determination method. Spectroscopy 5(8): 44-50.
36. Chen, C., L.S. Daley# and W.E. Kronstad. 1990. Photosynthetic
structure change in wheat cultivars during grain fill detected by in vivo
spectroscopy.  Plant Physiol. Biochem. 28(2): 259-269.
35. Daley#, L.S. 1990. French's nomenclature use: in vivo chlorophyll
protein complexes. Plant Physiol. Biochem. 28(2): 271-282.
34. Daley#, L. S., J.R. Powell, G.A. Strobel, R.B. Boone, J. Postman, M.
Willie and J. H. Brown. 1988. Spectroscopic methods for detection of
differences in photosynthetic apparatus and function of plants. Plant
Physiol. Biochem. 26: 683-694.
33. Jeong-B-R, L.S. Daley#, D.G. Smith, R.B. Boone and E.J. Zais. 1988.
Genetic modeling by spectros   copy of intact leaves of red-leafed hazel
(Corylus). Spectroscopy 3(2): 26-31.
32. Sanchez, E.E., R.A. Menendez, L.S. Daley#, R.B. Boone, O.L. Jahn and
P.B. Lombard. 1988. Charac   terization of quince (Cydonia) cultivars
using gradient polyacrylamide gel electrophoresis. J. Envi   ron. Hort. 6:
31. Jeong, B-R., L.S. Daley#, J. Postman, W.M. Proebsting and F.J.
Lawrence. 1988. Changes in chlo   rophyll-protein complexes associated
with June yellows of strawberry. Photochem. Photobiol. 47: 91-100.
 30. Daley#, L.S., J.M. Montano, R.A. Menendez, A.H. Soeldner and R.B.
Boone. 1987. Spectroscopic evaluation of freeze damage in pear trees using
fourth-derivative visible spectroscopy of bark sec   tions. Spectroscopy
2(9): 32-36.
29.Daley#, L.S., P.J. Breen and P. Mohanty. 1987. Measurement of
biological changes by fourth-deriva   tive visible spectroscopy of leaf
lamina. Spectroscopy 2(3): 32-35.
28. Daley#, L.S. and K.C. Nichols. 1987. Apparatus and computer program to
obtain activation energies of photosynthesis-example of studies in pear
varieties. Plant Physiology and Biochemistry (previously Physiologie
Vegetale) 25: 467-476.
27. Daley#, L.S., R.A. Menendez and R.L. Stebbins. 1987. Identification of
red- fruited pears by electro   phoresis and fourth derivative
spectroscopy  of intact lamina of pear leaves. J. Environ. Hort. 5: 25-
26. Ahmad, Z., L.S. Daley#, R.A. Menendez, and H.B. Lagerstedt. 1987.
Characterization of filbert (Corylus) species and cultivars using gradient
polyacrylamide gel electrophoresis. J. Environ. Hort. 5: 11-16.
25. Fisher, H. H., R.A. Menendez, L.S. Daley#, D. Robb-Spencer, and G.D.
Crabtree. 1987. Biochemical characterization of itchgrass (Rottboellia
exaltata) biotypes.  Weed Sci 35: 333-338.
24. Daley#, L.S., O. Jahn, and C. Guttridge. 1987. Differences between
Fragaria clones by fourth deriva   tive room temperature spectroscopy of
intact leaves. Photosynth. Res. 11: 183-188.
23. Guo, W.W., W.M. Proebsting, S.W. Potter, L.S. Daley, and J.R. Potter.
1987. Development of the alt mutant of pea Pisum sativum L. Plant
Physiology 85: 1089-1093.
22. Daley#, L.S. and L.J. Theriot. 1987. Proteins-similar to purothionin
from tomato (Lycopersicum esculenta), mango (Mangifera indica), papaya
(Carica papaya) and walnut (Juglans regia). Purification, protein redox
activity, and effect upon proteolytic activity. J. Agric. Food Chem. 35:
21. Daley#, L.S., M.M. Thompson, W.M. Proebsting, J. Postman, and B-R.
Jeong. 1986. Use of fourth- derivative visible spectroscopy of leaf lamina
in plant germplasm characterization. Spectroscopy 1(7): 28-31.
20. Daley#, L.S. 1986. Attenuance rank is a rapid non-extractive measure
of in vivo anthocyanin color and chlorophyll content. Scientia Hort. 28:
19. Menendez, R.A. and L.S. Daley#. 1986. Characterization of Pyrus
species and cultivars using gradient polyacrylamide gel electrophoresis.
J. Environ. Hort. 4: 56-60.
18. Amani, S., L.J. Theriot, and L.S. Daley#. 1985. An investigation of
some of the properties of biologi   cally significant phosphorylated
compounds as ligands of copper. Acta Chimica Inorganica 100: L23-L27 (the
letter L is from journal pagination).
17. Kim, S.K., H.B. Lagerstedt, and L.S. Daley#. 1985. Germination
responses of filbert pollen to pH, temperature, glucose, fructose and
sucrose. HortScience 20: 944-946.
16. Daley#, L.S. and G.A. Strobel. 1983. Galactofuranosidase activity in
Helminthosporium sacchari and its relationship to the production of
helminthosporoside. Plant Sci. Lett. 30: 145-154.
15. McGraw, T.L., L.A. Cates, L.S. Daley# and Y.C. Chen. 1981. Inhibitory
activity of the new adamantane derivative N,N' bis (ethylene)-P
(1-admantyl) phosphonic diamide against Rous sarcoma virus. Antiviral
Research 1: 269-272.
14. Vines, H.M., M.J. Donahue, L.R. Theriot, and L.S. Daley#. 1981.
Evolutionary divergence of plant mitochondria and sulphydryl reagent
sensitivity of grapefruit (Citrus paradisis MacF.) citrate synthase. Texas
J. Sci. 33: 159-168.
13. Theriot, L.R., R. Askins, C. Chang, L.S. Daley#, T. Davis, S. Gupta,
J. Talent, H. Tibbals and C. Wendel. 1981. Matrix effect in atomic
absorption determination of copper in spinach fraction I protein
preparation.  Possible role of copper in light sensitive control of
ribulose bisphosphate carboxylase activity. Texas J. Sci. 33: 235-251.
12. Fadare, S.O., L.S. Daley#, and Y.C. Chen. 1980. Guanosine reversal of
3- deazaguanidine inhibition of growth of rat kidney cells infected with
Rous sarcoma virus. IRCS Medical Science 8: 640-641.
11. Oncelay, C.Y., L.S. Daley#, H.M. Vines, G.A. Couvillon and C.H.
Hendershott. 1979. Seasonal fluctuations in dry weight, water content,
titratable acids, pH and respiration of dormant peach flower buds. Fruit
Sci. Reports 6: 163-171.
10. Oncelay, C.Y., L.S. Daley#, H.M. Vines, G.A. Couvillon and C.H.
Hendershott. 1979. Seasonal fluctuations in malate dehydrogenase,
phosphatase, proteinase activity of dormant peach (Prunus persica (L.)
Batsch.) flower buds. Scientia Hort. 11: 229-239.
9. Daley#, L.S., P. Carrol, and H. Mussell. 1979. The cell wall
phosphatase of cotton (Gossypium) is inhibited by Kelthane. Biochem. J.
179: 719-721.
8. Daley#, L.S., H.M. Vines and R.G.S. Bidwell. 1979. Oxidation of
phosphohydroxypyruvate and hydroxypyruvate: physiological implications in
plants. Canad. J. Bot. 57: 1-3.
7. Daley#, L.S., F. Dailey, and R.S. Criddle. 1978. Light activation of
RuBPCase: properties of the enzyme from tobacco Nicotianum tabacum. Plant
Physiol. 62: 718-722.
6. Spooner, J.S., L.S. Daley, G.O. Ware, and H.M. Vines. 1978.
Re-evaluation of cultural practices of jade (Crassula argentea (L.)
Thunberg.) J. Amer. Soc. Hort. Sci. 103: 306-308.
5. Daley#, L.S. and H.M. Vines. 1978. Pineapple (Ananas comosus (L.)
Merr.) leaf proteinase. Plant Sci. Lett. 11: 59-67.
4. Daley#, L.S., T.B. Ray, C.C. Black, and H.M. Vines. 1977.
Characteristics of phosphoenol-pyruvate carboxykinase form pineapple
leaves (Ananas comosus (L.) Merr.). Plant Physiol. 59: 618-622.
3. Daley#, L.S. and R.G.S. Bidwell. 1977. Separation of
phosphohydroxypyruvate, 3-phosphoglyceric acid and O-phosphoserine by
paper chromatography and chemical derivatization. J. Chromatog. 147:
2. Daley#, L.S. and H.M. Vines. 1977. Diurnal fluctuations of inorganic
orthophosphate in pineapple {Ananas comosus (L.) Merr.} leaves and a
possible role of ATPase. Plant Sci. Lett. 10: 289-298.
1. Daley#, L.S. and R.G.S. Bidwell. 1977. Phosphoserine and
phosphohydroxypyruvic acid. Evidence for their role as early intermediates
in photosynthesis. Plant Physiol. 60: 109-114.

NON-REFEREED PUBLICATIONS (Total 9,  number consecutively following
refereed publications to serve as reference list)
62. Kohnert, R.L., B.Arbogast, and L.S. Daley#. 1996. ACD/CNMR version
1.1. and C-13 NMR Module 1.2 for ChemWindows 3.1.3 or later: evaluation
and test for prediction of NMR spectra of photosynthetic C-13 metabolites.
Biotechnology Software (and Internet) Journal13(3) 17-23.
61. Eskelsen, S.R., G.D. Crabtree, R.B. Boone, and L.S. Daley#.  (1989)
Determination of herbicide damage to light harvesting apparatus of
radishes (Raphanus sativus). Proc. Western Soc. Weed  Sci. 42: 44- 51.
60. Daley#, L.S. 1985. Deuterium oxide interactions with: a) gibberellic
acid response expression; and b) mean June temperature at collection site,
in mint tissue culture. Current Topics in Biochemistry and Physiology 4:
230. (Minipaper)
59. Daley, L.S., N.S. Daley, and L.J. Theriot. 1983. Did mistletoe kill
Balder or was it the chemistry of visco   toxins? Review of chemical
properties and evaluation of probability. Hexagon 74: 87-90. (Review).
58. Daley#, L.S., J.E. Drumheller, M. Otte, S. Amani and L.J. Theriot.
1984. ESR of transition metal "chro   mophores" in RuBPCase. Supporting
neutron activation and optical data. Concepts of UV light activation
through transition metal chromophores. In H. Senger (ed) Blue Light
Effects in Biological Systems (Proceedings in Life Sciences),
Springer-Verlag, Berlin. pp. 156-169. (Symposium article).
57. Chen, Y.C., S. Fadare, F.J. Jenkins, and L.S. Daley#. 1980. Antiviral
activity of purine analogues of avian tumor viruses. Proc. IX.
International Symposia on Comparative Research on Leukemia and Relat   ed
Diseases. B. Lapin, ed.
56. Chen, Y.C., S. Fadare, F.J. Jenkins, and L.S. Daley#. 1980. Effects of
3-deazoguanine and virazole on Rous Sarcoma viruses. in: Advances in
Comparative Leukemia Research (1979). D.S. Yohn, B.A. Lapin, and J.R.
Blakeslee eds. Elsevier North-Holland. 1980. pp. 123-124.
55. Daley#, L.S., H.F. Tibbals and L.J. Theriot. 1980. Effect of 360 nm
light on RuBPCase products. In vitro role of copper on the reaction.  The
Blue Light Syndrome. H. Senger, ed. Springer-Verlag, Berlin, Heidelberg
and New York, pp. 381-391.

Patents U.S. Patent Application filed For: IMAGING SPECTROSCOPIC
INSTRUMENT AND METHODS (Li Ning, Gerald Edwards and Larry S. Daley).  OSU
Ref.No. 95-8; DOJ No. OSU-4-95, Attorney Ref. No. 245-44133/RJP).  This
patent is for an absorbance-spectrophotometric/fluorometric imaging device
to determine physiology, plant pathology, and water content and water
stress.  A second patent was also filed on a related instrument.


GRANT AND CONTRACTS  Prior to OSU: NSF (1981) Summer visiting Associate
Professorship, approximately $5,000 plus travel expenses, NSF (1982)
Summer Visiting Associate Professorship, approximately $7,500 plus travel
expenses, and DOD (1983) Summer Visiting Associate Professorship,
approximately, $8,000 plus travel expenses
Subtotal = $20,500
At OSU Support estimated at $264,821 has been generated in the last 13
years. Cooperative State Genetic Resources USDA-CSRS program support at
the National Clonal Germplasm Repository, Corvallis is not included in
total.  $52,091  Grant Oregon Wine Advisory Board  (Fingerprint and
characterize clones of Pyrus, Corylus, Fragaria, Mentha, Rubus, 1983-1988)
$105,600  Cooperative agreement with USDA (Research Agreement No
58-9AHZ-3-756, Evaluation and identification of genera, 1987-1991)
.$10,000  USDA cooperative grant  Scanning electron microscope study of
repository genera 58-0401-7-00154   $900 Travel funding, OECD (Acronym for
Organization of European Community Development) 200 pounds, other sources
$500 (1984) and was used to attend Blue Light Effects in Biological
Systems (Proceedings in Life Sciences), Marburg, Germany.  $2,000
Department of Energy (through Texas A&M Nuclear Engineering center), gave
approximately $2,000 since 1983, for neutron activation assays $70,000 The
Frasch foundation funded project "Spectroscopic non-invasive in field
monitor for status of plants"  (Cooperative venture. with J. B. Callis,
Univ. Washington, Seattle)   $4,000 Co-operative OECD Research Project
Funds supported a visit by Dr. Jack Farinoux (Senior French Plant
Physiologist) to work on advanced analysis of RuBPCase kinetics and
products in Sam Kent's laboratory, Dept. of Biochemistry and Microbiology,
University of Vermont. This work relates to theoretical mechanisms of
action of RuBPCase proposed by Daley and Kent. Costs cover travel to and
from France and living expenses for Dr. Farinoux. (Funded in French
Francs, est. $4,000).
Subtotal  external sources = $192,500

$20,197 Commission funding included: Oregon Nurserymen's Association:
6/01/93-12/31/95 Root weevil killing proteins; this includes $1,000
(1992-1993) and $8,100 (1993-1994) , $1,735 (1996-1997) for "Root weevil
kill test using viscotoxin plus protease inhibitor". $7,456 received in
1991-1993, for mass spectroscopic study "Finger printing Eastern Filbert
Blight" from the Oregon Filbert (now Hazelnut) Commission.  $18,506
(approximately 1,696 1991-1992; 2,560; 3,200 1992-1993; 5,050 1993-1994;
6,000 1994-1995) from the Filbert Commission for "Brownstain disorder:
insult and response an new approach to an old problem".$11,162 in from a
combination of the Organization of Oregon Nurserymen and the Oregon
Department of Agriculture in 1993-1994.
Subtotal commission funding $57,.321
Between 1983 and 1988 my lab received : $2,500 from Horticultural Research
Institute (with R. A. Menendez). for electrophoretic equipment; $700 from
Reynolds Aluminum in materials;  $200 from Sprague Electronics in
condensers; $300 from Sigma Chemical Co in reagents; and about $400 in
diverse items from General Electric, Isolab, Spectrum, 3M, etc.In 1989
Pharmacia donated a Phastgel apparatus worth about $8,000.  In 1993:
Johnson and Johnson donated glucose reaction strips worth approximately
$2,000.  In 1995 Lucidyne donated a color printer valued at about $900.
Subtotal = $15,000
Total funding = $264,821